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【英文标准名称】:StandardPracticeforDetectionofMycoplasmaContaminationofCellCulturesbyUseofBisbenzamideDNA-BindingFluorochrome
【原文标准名称】:用二苯甲酰胺与DNA结合荧光染料法检测细胞培养中支原体污染物的标准实施规程
【标准号】:ASTME1532-2000(2006)
【标准状态】:现行
【国别】:
【发布日期】:2000
【实施或试行日期】:
【发布单位】:美国材料与试验协会(US-ASTM)
【起草单位】:E48.02
【标准类型】:(Practice)
【标准水平】:()
【中文主题词】:医学科学;微生物学;验证
【英文主题词】:cellculture;DNAstain;indicatorcells;mycoplasma
【摘要】:Mycoplasmahyorhinis,cultivarx03B1;strains(1)3donotgrowonanyofthestandardmediausedformycoplasmacultivation.Thesestrains,whicharefoundascontaminantsincellcultures,aredetectedbyindirectmethods.Aspecializedmediumhasbeendescribedbutitisnotyetinwideuse(2).ThispracticeshouldbeusedinconjunctionwithPracticeE1531.Allcellculturestobeexaminedformycoplasmashouldundergoaminimumoftwopassagesinantibiototic-freetissueculturemediumbeforetesting.1.1ThispracticecoverstheuseofcellculturesandDNA-bindingflurorochrometechniquestodetectmycoplasmacontaminationofcellcultures.1.2Thispracticedoesnotcoveraxeniccultivationoridentificationofmycoplasmas.Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappropriatesafetyandhealthpracticesanddeterminetheapplicabilityofregulatorylimitationspriortouse.
【中国标准分类号】:C05
【国际标准分类号】:07_100_10
【页数】:2P.;A4
【正文语种】: